Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Identifieur interne : 003A06 ( Main/Exploration ); précédent : 003A05; suivant : 003A07Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.
Auteurs : L J Lipinski [États-Unis] ; H L Ross ; B. Zajc ; J M Sayer ; D M Jerina ; A. DippleSource :
- International journal of oncology [ 1019-6439 ] ; 1998.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, DNA Adducts, DNA Polymerase I, Deoxyguanosine, Oligonucleotides.
- DNA Replication, Mutation, Templates, Genetic.
Abstract
Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.
DOI: 10.3892/ijo.13.2.269
PubMed: 9664121
Affiliations:
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Zajc</name></author><author><name sortKey="Sayer, J M" sort="Sayer, J M" uniqKey="Sayer J" first="J M" last="Sayer">J M Sayer</name></author><author><name sortKey="Jerina, D M" sort="Jerina, D M" uniqKey="Jerina D" first="D M" last="Jerina">D M Jerina</name></author><author><name sortKey="Dipple, A" sort="Dipple, A" uniqKey="Dipple A" first="A" last="Dipple">A. Dipple</name></author></analytic><series><title level="j">International journal of oncology</title><idno type="ISSN">1019-6439</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide (metabolism)</term><term>DNA Adducts (metabolism)</term><term>DNA Polymerase I (metabolism)</term><term>DNA Replication</term><term>Deoxyguanosine (metabolism)</term><term>Mutation</term><term>Oligonucleotides (metabolism)</term><term>Templates, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol (métabolisme)</term><term>Adduits à l'ADN (métabolisme)</term><term>DNA polymerase I (métabolisme)</term><term>Désoxyguanosine (métabolisme)</term><term>Matrices (génétique)</term><term>Mutation</term><term>Oligonucléotides (métabolisme)</term><term>Réplication de l'ADN</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide</term><term>DNA Adducts</term><term>DNA Polymerase I</term><term>Deoxyguanosine</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>7,8,8a,9a-Tétrahydro-benzo[10,11]chryséno[3,4-b]oxirène-7,8-diol</term><term>Adduits à l'ADN</term><term>DNA polymerase I</term><term>Désoxyguanosine</term><term>Oligonucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Replication</term><term>Mutation</term><term>Templates, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Matrices (génétique)</term><term>Mutation</term><term>Réplication de l'ADN</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Dipple, A" sort="Dipple, A" uniqKey="Dipple A" first="A" last="Dipple">A. Dipple</name><name sortKey="Jerina, D M" sort="Jerina, D M" uniqKey="Jerina D" first="D M" last="Jerina">D M Jerina</name><name sortKey="Ross, H L" sort="Ross, H L" uniqKey="Ross H" first="H L" last="Ross">H L Ross</name><name sortKey="Sayer, J M" sort="Sayer, J M" uniqKey="Sayer J" first="J M" last="Sayer">J M Sayer</name><name sortKey="Zajc, B" sort="Zajc, B" uniqKey="Zajc B" first="B" last="Zajc">B. Zajc</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Lipinski, L J" sort="Lipinski, L J" uniqKey="Lipinski L" first="L J" last="Lipinski">L J Lipinski</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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